Glycoprotein Biosynthesis in Phytophthora megasperma f. sp. glycinea, An in vitro Study

Glycoprotein Biosynthesis, Race Specific Determinants, Phytophthora megasperma f. sp. glycinea, Lipid-Intermediates, Tunicamycin, Diumycin Extracellular glycoproteins have been claimed to represent the specificity determinants within different races o f Phytophthora megasperma f. sp. glycinea (formerly P. m. var. sojae) specifically infective in different soybean cultivars. Microsomal preparations o f three different races o f Pmg are capable o f synthesizing both N-glycosidically linked oligosaccharides and short O-glycosidically linked oligomannoses in vitro from corresponding sugar nucleotides. In addition, lipidic glycosidic intermediates (dolichol type derivatives) serve as specific glycosyl donors. In general, the results demonstrate that the well-known ubiquitous biosynthetic pathway o f asparagine-di-Nacetyl-chitobiose type glycoproteins is established in that fungus. The significance o f the results regarding specific elicitors, suppressors, protectants, and extracellular glycoenzym es is discussed.